Achaea janata

mRNA-seq of A. janata midgut_Toxin_tolerant_replicate

Library was prepared using Illumina TruSeq stranded mRNA Library Preparation Kit and as per its described protocol. Briefly, mRNA was enriched from total RNA followed by fragmentation. The fragmented mRNA was converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation and finally ended by limited number of PCR amplification of the adaptor-ligated libraries. Library quantification and qualification was performed using DNA High Sensitivity Assay Kit.

{'age': '3rd Instar', 'temp': '25', 'tissue': 'Mid-gut', 'altitude': '505m', 'geo_loc_name': 'India:Hyderabad, Deccan Plateau', 'biosamplemodel': 'Invertebrate', 'collection_date': '2015-03-25', 'isolation_source': 'Cry toxin exposed larva', 'biomaterial_provider': 'Prof. Aparna Dutta-Gupta; Laboratory of Insect Molecular Biology'}

mRNA-seq of A. janata fatbody

Library was prepared using Illumina TruSeq stranded mRNA Library Preparation Kit and as per its described protocol. Briefly, mRNA was enriched from total RNA followed by fragmentation. The fragmented mRNA was converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation and finally ended by limited number of PCR amplification of the adaptor-ligated libraries. Library quantification and qualification was performed using DNA High Sensitivity Assay Kit.

{'age': '1st to 5th Instar', 'temp': '25', 'tissue': 'Fatbody', 'isolate': '1st to 5th Instar (pooled sample)', 'altitude': '505m', 'dev_stage': 'Larval', 'collected_by': 'Vinod K. Chauhan', 'geo_loc_name': 'India:Hyderabad', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-09-15', 'isolation_source': 'Deccan Plateau', 'biomaterial_provider': 'Prof. Aparna Dutta-Gupta; Laboratory of Insect Molecular Biology'}

mRNA-seq of A. janata fatbody

Library was prepared using Illumina TruSeq stranded mRNA Library Preparation Kit and as per its described protocol. Briefly, mRNA was enriched from total RNA followed by fragmentation. The fragmented mRNA was converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation and finally ended by limited number of PCR amplification of the adaptor-ligated libraries. Library quantification and qualification was performed using DNA High Sensitivity Assay Kit.

{'age': '1st to 5th Instar', 'temp': '25', 'tissue': 'Fatbody', 'isolate': '1st to 5th Instar (pooled sample)', 'altitude': '505m', 'dev_stage': 'Larval', 'collected_by': 'Vinod K. Chauhan', 'geo_loc_name': 'India:Hyderabad', 'biosamplemodel': 'Invertebrate', 'collection_date': '2018-09-15', 'isolation_source': 'Deccan Plateau', 'biomaterial_provider': 'Prof. Aparna Dutta-Gupta; Laboratory of Insect Molecular Biology'}