Ascosphaera

fungal mycelia

Ascosphaera apis mycelium sample is termed as Aam

{'host': 'not collected', 'isolate': 'not collected', 'sample_type': 'fungal mycelia', 'geo_loc_name': 'not collected', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': 'not collected', 'isolation_source': 'not collected', 'biomaterial_provider': 'Honeybee Protection Group, Honeybee-Pathogen Interaction Mechanism Research Team, College of Bee Science'}

fungal mycelia

Ascosphaera apis mycelium sample is termed as AamL

{'host': 'not collected', 'strain': 'not collected', 'isolate': 'not collected', 'altitude': 'not collected', 'sample_type': 'fungal mycelia', 'geo_loc_name': 'not collected', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': 'not collected', 'isolation_source': 'not collected', 'biomaterial_provider': 'Honeybee Protection Group, Honeybee-Pathogen Interaction Mechanism Research Team, College of Bee Science'}

Ascosphaera apis mycelium

Total RNA of Ascosphaera apis mycelium sample isolated using TRizol reagent (Thermo Fisher, Shanghai, China) on dry ice and thenreversely transcribed using Maxima H Minus Reverse Transcriptase, followed by purification and concentration on a AMPure XP beads. Secondly, cDNA librarieswere constructed from 50 ng total RNA with a 14 cycles PCR using the cDNA-PCR Sequencing Kit (SQK-PCS109) and PCR Barcoding Kit (SQK-PBK004) followingthe manufacturer's protocol (Oxford Nanopore Technologies Ltd, Oxford, UK). Thirdly, the aforementioned cDNA libraries were sequenced by BiomarkerTechnologies (Beijing, China) utilizing PromethION system (Oxford Nanopore Technologies Ltd, Oxford, UK).

{'host': 'Apis mellifera ligustica', 'strain': 'Fuzhou strain', 'isolate': 'Fuzhou isolate', 'sample_type': 'whole organism', 'geo_loc_name': 'China: Fuzhou', 'biosamplemodel': 'Microbe, viral or environmental', 'collection_date': '2018-10-10'}